Analysis performed by the NRC
- Confirmation of bacterial identification to species level using MALDI-TOF MS
- In vitro susceptibility testing to antimicrobial drugs by disc diffusion according to EUCAST guidelines
- Determination of MICs by broth microdilution method according the EUCAST guidelines and interpretative breakpoints
- Carbapenem hydrolysis-based tests
- Inhibitors-based combination disk tests:
- For the detection of ESBL and/or hyperproduced AmpC
- For the detection of carbapenemase
- Extended susceptibility disk diffusion testing against aminoglycosides for the detection of 16S rRNA methylases
- Immunochromatographic detection of carbapenemase (OXA-48, KPC, VIM, NDM and OXA-23)
- End-point PCR simplex or multiplex targeting
- Class A and class B carbapenemases (NDM, VIM, KPC, IMP, IMI, SIM, GIM, DIM, FIM, SMB, SME, SPM)
- Class D carbapenemases (OXA-48, OXA-427, OXA-198, OXA-23, OXA-24, OXA-58)
- Major ESBL families (TEM, SHV, CTX-M group 1, 2 and 9 )
- minor ESBL families (GES, VEB, PER and BEL)
- plasmidic cephalosporinases (MOX, CMY, DHA, ACC, ACT/MIR, FOX)
- oxacillinases groups (OXA-1,OXA-2,OXA-9,OXA-10, OXA-18,OXA-20)
- Plasmid-mediated colistin resistance (MCR-1, MCR-2, MCR-3, MCR-4, MCR-5)
- 16S rRNA methylases (ArmA, Rmt A-B-C-D-E-F-G, NpmA)
- plasmid-borne quinolone resistance genes (QnrA, QnrB, QnrS)
- Other genetic characterization methods:
- Sequencing of resistance genes and the genetic environment by NGS
- Plasmid extraction by Kieser method
- Conjugation, transformation and cloning
- PCR-based replicon typing : method based on replicons (Inc/rep PCR) of the major plasmid incompatibility groups (Caratolli A. et al , JAC. 2010 Dec;65(12):2518-29)
- Multiplex End-point PCR detecting capsular types (K1, K2, K5, K20, K54 and K57) and virulence factors (rmpA, wcaG, terW, iutA, repA and silS) in K.pneumoniae
- Quadruplex End-point PCR assigning E.coli to the following phylo-groups (A,B1,B2,C,D,E and F)
